Peptide Dosage & Reconstitution Calculator

Peptides are supplied as a lyophilized (freeze-dried) powder in a sterile vial. Before use, the powder must be reconstituted (dissolved) in bacteriostatic water (BAC water). This process is straightforward but must be done carefully to preserve the peptide.

Step-by-step process

1. Gather supplies: Your peptide vial, bacteriostatic water, an alcohol swab, and a syringe (typically 1 mL insulin syringe).
2. Clean the vial tops: Wipe both the peptide vial and BAC water vial tops with an alcohol swab.
3. Draw bacteriostatic water: Using the syringe, draw your chosen amount of BAC water. Common amounts are 1 mL, 2 mL, or 2.5 mL. More water means a more dilute solution (easier to measure small doses).
4. Add water to the peptide vial: Insert the needle into the peptide vial and slowly release the water down the inside wall of the vial. Do not spray directly onto the powder, as this can damage the peptide.
5. Gently mix: Swirl the vial gently until the powder is fully dissolved. Do not shake vigorously. The solution should be clear.
6. Store properly: Reconstituted peptides should be refrigerated (2-8 degrees C) and typically used within 28-30 days.

Understanding insulin syringes

Standard insulin syringes hold 1 mL (100 units). Each small tick mark represents 1 unit (0.01 mL). A half-syringe is 50 units (0.5 mL). When the calculator shows "10 units," that means drawing the syringe to the 10 mark, which equals 0.1 mL of liquid.

Why the amount of water matters

Adding more water makes the solution more dilute, which makes it easier to measure small doses accurately. Adding less water makes the solution more concentrated, meaning you inject a smaller volume per dose. Neither approach changes the total amount of peptide in the vial.